Poly Pro Water Baths

Water Baths

Revolutionary Science is a long standing manufacturer of digital and analog water baths, since 2003, known as the Revolutionary Science Pro Bath line. The Poly Pro Baths range in size and sophistication, including Wi-Fi capability and dual chamber technology. The polypropylene liners chemically resistant and an excellent choice for applications, where bleach or other chemicals may be added to the water to prevent cross contamination. Stainless steel baths can pit, corrode and scale evn under normal use as class I distilled water will pull ions out of the stainless chamber. Corrosion does not occur with polypropylene water baths.

Common applications for water baths are DNA extraction, protein purification, bacteria transformation, CRISPR, agar and agarose melting and thawing embryos, sperm and vaccines. The Mini Pro Bath is the only Revolutionary Science water bath that is analog and is equipped with an aluminum chamber instead of polypropylene for rapid heating applications, like boiling agar or agarose.

water bath applications

Area of Focus

Microbiology

Food/Beverage

Bacteriology

Pharmaceutical

Molecular Biology

Applications

Warm media

Thaw sperm & embryos

Thaw vaccine

Bacterial trans/heat shock

Cell culture

LPC tests

PCR sample prep

Acid degree value test – dairy

DNA extraction

Protein purification

Protein denaturing

Digestions

Cell lysing

enzymatic & serologic

Transform bacterial DNA through methods using heat shock and recombination

Bacterial (prokaryotic) chromosomes are in the form of hoops and circles and are far smaller than eukaryotic chromosomes. By forcing a plasmid (circular strand of foreign DNA) to enter a bacterial cell, the plasmid can merge into the chromosome can be change the DNA. This process is called transformation. Bacterial cells and the plasmid of interest can be stored in a warm environment (most likely at 37C). A solution can be stored in a Revolutionary Science Incufridge and Poly Pro Bath. During this incubation period, the cell walls become porous and allow the plasmid to enter. Solution can then be transferred to a water bath at 42 degrees Celsius. The abrupt change in temperature will “heat shock” the solution. When the cell is exposed to the elevated temperature, the lipid membrane closes and holds in the plasmid, allowing it to insert itself into the chromosome and reprogram it. Bacterial cells can be inoculated on a petri plate, incubated in an incubator, where colony forming units (CFU’s) can be counted either by hand or with an automated colony counter, like the IncuCount. Aseptic techniques should be employed. Petri plates should be properly disposed of after they are no longer needed, by sterilizing in an autoclave, like the Saniclave 50.

Isolate and extract DNA from crude samples for analysis

Prior to amplification or analysis of DNA, the DNA must be extracted from the tissue sample. A small tissue sample of an organism can be ground and pulverized by hand using a pestle and a micro centrifuge tube. This pulverized sample is now referred to as the crude sample. This process continues by rupturing and denaturing the cell wall (composed of a lipid membrane and proteins), called lysing. Cells are lysed with lysis solution and exposed to elevated temperatures in two water baths, like the Poly Pro Bath or Dual Poly Pro Bath. A micro centrifuge, like a RevSpin and vortexer are also used in the process to remove the lipids and proteins from the mixture. When the process is complete, the DNA chromosomes can be viewed under a microscope and is ready to be amplified in a PCR machine like the RevCycler or analyzed through DNA electrophoresis in a DNA RevBox or even sequenced.

Purify proteins from crude samples

Prior to analysis of proteins by means of protein electrophoresis or spectrophotometry, the DNA and lipids must be removed from the tissue sample. A small tissue sample of an organism can be ground and pulverized by hand using a pestle and a micro centrifuge tube. This pulverized sample is now referred to as the crude sample. This process continues by rupturing and denaturing the cell (composed of DNA, carbohydrates, lipid membrane and proteins) is called lysing. Cells are lysed with lysis solution and exposed to elevated temperatures in two water baths, like the Poly Pro Bath or Dual Poly Pro Bath. A micro centrifuge, like a RevSpin and vortexer are also used in the process to remove the lipids, sugars and DNA from the mixture. When the process is complete, the proteins remain. Proteins, depending on their abundance can be analyzed through protein electrophoresis in a Protein RevBox, spectrophotometry or even liquid chromatography and mass spectrometry.