To transform bacteria, a foreign plasmid (circular DNA) is introduced into the cell, where it can integrate into the bacterial chromosome. The process involves storing bacteria and the plasmid in a warm environment (around 37°C) to make the cell walls porous. The solution is then "heat shocked" in a water bath at 42°C, causing the cell membrane to close and trap the plasmid inside. Once transformed, the cells can be grown on petri plates in an incubator, and the resulting colonies can be counted. Aseptic techniques should always be used. Afterward, plates should be sterilized in an autoclave, like the Saniclave 50, for proper disposal.

Before DNA can be analyzed, it must be extracted from a tissue sample. The sample is first pulverized to create a crude mixture. The cells are then lysed (ruptured) using a lysis solution and heat in a water bath, like the Poly Pro Bath or Dual Poly Pro Bath. A micro centrifuge, such as a RevSpin, and a vortexer are used to separate the DNA from lipids and proteins. Once isolated, the DNA is ready for amplification in a PCR machine, analysis via electrophoresis in a DNA RevBox, or sequencing.

Before protein analysis, DNA and lipids must be removed from a tissue sample. The tissue is first ground into a crude sample. The cells are then lysed using a lysis solution and heat in two water baths, like the Poly Pro Bath or Dual Poly Pro Bath. A micro centrifuge, such as a RevSpin, and a vortexer are used to remove DNA, lipids, and carbohydrates from the mixture, leaving the purified proteins. These proteins can then be analyzed using techniques like protein electrophoresis in a Protein RevBox, spectrophotometry, or mass spectrometry.

Whether you're performing DNA extraction, protein purification, or cell pelleting, a reliable microcentrifuge is essential for your lab. Our RevSpin line offers powerful, cost-effective options trusted by researchers and even featured on TV shows like CSI Miami. Have questions about choosing between the RevSpin 102 and RevSpin 200, or want to learn more about how our microcentrifuges can improve your specific applications?